First Molecular Characterization of Cryptosporidium spp. in Patients Living with HIV in Honduras

Cryptosporidiosis is one of the most important causes gastroenteritis in world, especially low- and middle-income countries. It caused by Apicomplexan parasite Cryptosporidium spp., mainly affects children immunocompromised people, whom it can pose a serious threat to their health, or even be life threatening. In Honduras, there are no data on species molecular diversity subtypes. Therefore, cross-sectional study was conducted between September 2019 March 2020 for identification spp. 102 patients living with HIV who attended national hospital Tegucigalpa. Stool samples were analyzed direct microscopy, acid-fast stained smears, rapid lateral flow immunochromatographic test. All that tested positive molecularly identify subtype using three different markers: gp60, cowp, 18Sr. PCR products also sequenced. Four out (3.92%) Cryptosporidiumparvum, all assigned IIa. These findings suggest possible zoonotic transmission this population.